LUNGENE Rapid Test Kit (Packet of 25 Test Kits)

A. Prepare the Reagents

• Allow the test devices, reagents and specimens to equilibrate to room temperature (15-30°Cor 59-86°F) prior to testing.
• Put an extraction tube on the work station.
• Unscrew the lid of an extraction reagent. Add all of the extraction reagent into an extraction tube.

B. Swabbing

You can collect both Nasopharyngeal & Oropharyngeal Samples or Just 1

Nasopharyngeal Swab Specimen Collection

1. Remove the swab from the package.
2. Tilt patient’s head back about 70°.
3. Insert the swab through the nostril parallel to the palate (not upwards) until resistance is encountered or the distance is equivalent to that from the ear to the nostril of the patient, indicating contact with the nasopharynx. (Swab should reach depth equal to distance from nostrils to outer opening of the ear.) Gently rub and roll the swab. Leave swab in place for several seconds to absorb secretions.
4. Slowly remove swab while rotating it.

Specimens can be collected from both sides using the same swab, but it is not necessary to collect specimens from both sides if the mini tip is saturated with fluid from the first collection. If a deviated septum or blockage creates difficulty in obtaining the specimen from one nostril, use the same swab to obtain the specimen from the other nostril.

Oropharyngeal Swab Specimen Collection

Insert swab into the posterior pharynx and tonsillar areas. Rub swab over both tonsillar pillars and posterior oropharynx and avoid touching the tongue, teeth, and gums.

C. Test Procedure

1. Insert the swab specimen into the extraction tube which contains extraction reagent. Roll the swab at least 5 times while pressing the head against the bottom and side of the extraction tube. Leave the swab in the extraction tube for one minute.
2. Remove the swab while squeezing the sides of the tube to extract the liquid from the swab. The extracted solution will be used as test sample.
3. Cover the extraction tube with a dropper tip tightly.
4. Remove the test cassette from the sealed pouch.
5. Reverse the specimen extraction tube, holding the tube upright, transfer 3 drops (approximately 100μL) slowly to the specimen well(S) of the test cassette, then start the timer.
6. Wait for colored lines to appear. Interpret the test results at 15 minutes.